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Initial observation took place when we obtained sera from 18 patients with ACS and analyzed them for presence of C. pneumoniae specific IgG and IgA using ELISA Medac kit (Germany) as well as for presence of genomic determinants of C. pneumoniae. As can be seen from Table 1, 7 patients from the initial group were positive for C. pneumoniae-specific IgG, whereas 4 patients had diagnostically relevant levels of IgA. Simultaneous detection of increased titers of IgG and IgA was documented only in 4 patients. Surprisingly, when DNA specimens extracted from 1.0 ml of serum aliquots were analyzed for presence of 16S rRNA by conventional PCR, we have found that 5 patients with ACS were positive for the genetic marker of C. pneumoniae. Finally, just 3 patients (out of 18) had increased levels of two Ig isotypes and positive signal in conventional PCR for 16S rRNA. Such inconsistency between serologic and genetic markers of C. pneumoniae infection is well known and widely discussed (1). However, detectability of the genetic marker of C. pneumoniae in human serum appeared to be a reasonably intriguing finding. Therefore, we decided to optimize conventional PCR protocol for detection of the C. pneumoniae genetic markers in serum.. history and external environmental factors like natural selection, as if.

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Following introduction of MCC, 1183 OHCA patients were treated from November 1, 2007, to December 31, 2011 (period 2). They were compared with 1218 OHCA patients before MCC was introduced from January 1, 1998, to May 30, 2003 (period 1). Patients in period 2 were evaluated for survival in relation to MCC use..